Comparison of the PREMIER cryptococcal antigen enzyme immunoassay and the latex agglutination assay for detection of cryptococcal antigens
نویسندگان
چکیده
منابع مشابه
Comparison of the PREMIER cryptococcal antigen enzyme immunoassay and the latex agglutination assay for detection of cryptococcal antigens.
A new enzyme immunoassay (EIA), PREMIER Cryptococcal Antigen, was compared with latex agglutination (LA) for the detection and quantitation of circulating capsular polysaccharide antigen from Cryptococcus neoformans. The clinical evaluation of PREMIER EIA as a screening assay, including 475 specimens with 120 LA and EIA positives, resulted in 99% sensitivity and 97% specificity. The clinical sp...
متن کاملEffect of potential interference factors on performance of enzyme immunoassay and latex agglutination assay for cryptococcal antigen.
The PREMIER Cryptococcal Antigen Enzyme Immunoassay (Meridian Diagnostics) did not give discrepant results with rheumatoid factor, syneresis fluid, or serum macroglobulins from systemic lupus erythematosis patients. The Cryptococcal Antigen Latex Agglutination System (Meridian Diagnostics) did cross-react with syneresis fluid but not with the other serum factors tested.
متن کاملComparison of three commercial cryptococcal latex kits for detection of cryptococcal antigen.
Three commercial latex kits, IBL, MYCO-Immune, and IMMY, for the detection of cryptococcal antigen were compared in regard to sensitivity, specificity, and height of antigen titers. A total of 218 cerebrospinal fluid and 79 serum specimens from 239 patients were included. Twenty-two patients had culture-proven disseminated cryptococcosis. Both the IBL and MYCO-Immune kits had sensitivities of 1...
متن کاملComparison of four assays for the detection of cryptococcal antigen.
We compared the performance of four assays for the detection of cryptococcal antigen in serum samples (n = 634) and cerebrospinal fluid (CSF) samples (n = 51). Compared to latex agglutination, the sensitivity and specificity of the Premier enzyme immunoassay (EIA), Alpha CrAg EIA, and CrAg lateral flow assay (LFA) were 55.6 and 100%, 100 and 99.7%, and 100 and 99.8%, respectively, from serum sa...
متن کاملNew cause for false-positive results with the cryptococcal antigen test by latex agglutination.
The highly specific and sensitive latex agglutination test for cryptococcal antigen detection in cerebrospinal fluid is routine in many hospitals. Contamination of cerebrospinal fluid by a minute amount of syneresis fluid (surface condensation) from agar gave a strongly positive reaction which was heat stable, was not eliminated by pronase treatment, and was not detected by the normal rabbit gl...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1991
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.29.8.1616-1619.1991